Entinostat reverses P-glycoprotein activation in snail-overexpressing adenocarcinoma HCC827 cells.

Entinostat reverses P-glycoprotein activation in snail-overexpressing adenocarcinoma HCC827 cells.

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Epithelial-to-mesenchymal transition (EMT) in cancer cells facilitates tumor progression by promoting invasion and metastasis.Snail is a transcriptional factor that induces EMT, while P-glycoprotein (P-gp) is an efflux transporter involved in anticancer drug resistance, and P-gp efflux activity is stimulated in Snail-overexpressing lung cancer cells with EMT characteristics.Since the histone deacetylase (HDAC) inhibitor entinostat (Ent) reverses EMT features, our aim in this study was to determine whether Ent also suppresses P-gp activation in Snail-induced cells.

First, we confirmed that Ent treatment reduced migration activity, downregulated E-cadherin and upregulated vimentin at the mRNA level in Snail-overexpressing cells, thus Urn Necklace Gift Box inhibiting EMT.Efflux and uptake assays using rhodamine123 (Rho123), a fluorescent P-gp substrate, showed that Ent also inhibited Snail-induced activation of P-gp.Moreover, P-gp activity was more strongly inhibited by Ent in Snail-overexpressing cells than in Mock cells.

When we evaluated the uptakes of Rho123 by LLC-PK1 cells and P-gp-overexpressing LLC-GA5COL150 cells, Rho123 accumulation in LLC-GA5COL150 cells was significantly decreased compared with that in LLC-PK1 cells.Coincubation with Ent had no effect on Rho123 accumulation in either of the cell lines.Thus, Ent appears to be an inhibitor, but not a substrate, of P-gp at low concentration.

Our results suggest that Ent UltraTemp Parts treatment might suppress not only Snail-induced cancer malignant alteration, but also P-gp-mediated multidrug resistance.